Two possible approaches. One is remove by adsorption to a matrix like Kieselguhr or use a solvent system in TLC that will retain the chlorophyl at the origin while allowing your other components to migrate. You will also have to use different types of plates. The other option is to use an HPLC system to optimize the conditions for removing this component.

Hi,

Mr Nair thanks for your suggestion . i ll try this .

Beside other suggestion, you are able to use biological method to remove chlorophyll. Since acidic bacteria such as Lactobacillus lactis has potential to remove that for you.

I think that the bacteria might metabolize the other organic compounds present in the plant extract resulting in the production of organic acids in the medium. as by products

what about using charcoal for adsorbing that chlorophyll.

I did by charcoal already and its showing quite good result but its not remove completely , and biological method wont be a good idea i think so , but still let me try it once.

try again

i put activated charcoal with Ethyl acetate solvent in my extract and kept it for 2 days and now i got a complete clear solution.  i think not only chlorophyll my other components also absorbed by charcoal.

can anybody suggest that in what ratio and the time duration of charcoal treatment for chlorophyll removal process.  

Mr Swain you may remove all the adsorbed components using polar organic solvent like methanol or acetone from the charcoal by simple treatment.  Then again treat the extract with charcoal for shorter duration making chlorophyll to be adsorbed.