How can i record my sequence(nucleotide) submitted to the ncbi(bankit)?

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How to solve forrtl:severe (408): fort (10): subscript#1 of the array IDOP error in SWAT?

Dear SWAT user I faced the attached error when I ran SWAT 2012 in ArcGIS 10.5. I already looked at the swat2012.mdb to solve the problem but unfortunately couldn't. Does anyone know how I can...

21 August 2023 7,250 3 View

How can I solve the error (72): floating overflow in ArcSWAT?

I'm using ArcSWAT for GIS 10.5 and when I ran my model I get the error (72): floating overflow. Can you help me how to fix the problem Best regard mahsima

04 December 2022 3,465 1 View

What's the number of outlet and the number of subbasin aren't equal in watershed delineation?

I'm using Arcswat for GIS 10.2 and when delineating the watershed the number of outlets and the number of subbasins aren't equal but when I look at the topographical report their equal, for...

07 October 2022 5,407 2 View

How to change POSCAR file after doping?

I want to calculate diffusion coefficient for a material after doping using VASP but I don't know how to change POSCAR file. what changes should I put in POSCAR file for Li10‑xSnP2S12‑xClx and...

23 April 2022 1,835 1 View

How can I download the VIPER Tools software package for ENVI 5.3 ?

Recently I am trying to download the VIPER Tools software package for ENVI 5.3 but I can't because the original Viper Tools website (www.vipertools.org) is no longer operational. So How can I...

08 January 2022 1,325 3 View

How can identify element number(or index) in ABAQUS scripting instead of using mask?

Dear all I want to specify random material to elements. for doing this first of all I want to create sets to elements then specify the materials tho them. to create sets in script we should...

05 September 2020 7,144 6 View

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

13 May 2020 1,851 6 View

How can I digest my recombinant plasmid with xho1 and hind3 enzyms?

Hi, I have tried to digest my recombinant plasmid( PeGFP plasmid 4700 bp and fragment 750 bp) with the following protocol: 1-template= 3 (micro) xho1 =1.5 Hind3=2 buffer=3 H2O=20.5 1 hour in...

13 May 2020 5,410 3 View

In an optical diffraction (single slit experiment) we found no photons in dark fringes that should interfere destructively, how this is possible?

we did a very simple experiment of optical diffraction (single slit experiment) with a laser diode and observed dark-bright fringes on the plane of diffraction experiment (details of experiment...

13 March 2020 3,471 3 View

How can i model & simulate stress distribution at coating-substrate interface with different interface texture?

saying texture i mean topography.

12 March 2020 8,522 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

How do I run a dead well test for a QuantStudio 5 qPCR machine?

I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...

24 July 2024 8,172 1 View