How can identify element number(or index) in ABAQUS scripting instead of using mask?

Dear all I want to specify random material to elements. for doing this first of all I want to create sets to elements then specify the materials tho them. to create sets in script we should...

05 September 2020 6,679 6 View

How can I improve my protein purification with Qiagen protocol-Ni-NTA column?

Hi, I purified a protein (about 28KD) via the following steps: 1- Firstly, I used buffer A native(Qiagen)-NaH2PO4,NACL,Immidazol,ph8 2-then after centrifuging and separating my pellet , I added...

13 May 2020 1,673 6 View

How can I digest my recombinant plasmid with xho1 and hind3 enzyms?

Hi, I have tried to digest my recombinant plasmid( PeGFP plasmid 4700 bp and fragment 750 bp) with the following protocol: 1-template= 3 (micro) xho1 =1.5 Hind3=2 buffer=3 H2O=20.5 1 hour in...

13 May 2020 5,153 3 View

In an optical diffraction (single slit experiment) we found no photons in dark fringes that should interfere destructively, how this is possible?

we did a very simple experiment of optical diffraction (single slit experiment) with a laser diode and observed dark-bright fringes on the plane of diffraction experiment (details of experiment...

13 March 2020 3,198 3 View

How can i model & simulate stress distribution at coating-substrate interface with different interface texture?

saying texture i mean topography.

12 March 2020 8,161 3 View

How to know abaqus and fortran are linked?

Hi. I have a technical question. I linked abaqus and fortran by a tutorial step by step and my supervisor told me to check it (successful linked) from abaqus command and I just don't know how to...

02 May 2019 438 2 View

How to find out effect of heat treatments on mechanical properties of materials ( like AISI D2 )?

Hi everybody; As you know material's mechanical properties have changed after heat treatments. So each one of you know how to find a way to reach the relation between heat treatments and for...

14 February 2019 8,251 7 View

What is cause of the difference between numerical stress-strain curve and the experimental results of the split hopkinson pressure bar in abaqus?

Hi I have simulated the SHPB test based on the attached image conditions in Abaqus (3D and one quarter model). The analysis is dynamic/explicit. The bars are linearly elastic. The cylindrical...

21 May 2018 3,555 4 View

SEM error after sample coating (height recognition)?

I am trying to do NOVA FEG-SEM on a few PDMS samples which are Iridium coated (thickness roughly 10 nm). Every time that I bring the sample surface (stub) up to 15mm, the Z-direction re-linking...

28 August 2017 8,194 3 View

How we can use dll files instead of mex files in s-functions in matlab?

07 November 2016 3,724 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View