How can I recognize nanogels visually?

More Robin Rajan's questions See All

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Protura for dummies?

Is it possible to tell Proturan families apart based on overall appearance? All the keys I have seen are based on very fine characteristics only readily visible on a slide. Also, there seem to be...

14 June 2024 6,334 0 View

Is it necessary to perform both forward and reverse Sanger sequencing to verify the presence of a gene in a sample?

Hello :) I am looking at the presence of toxin biosythensis genes in lake sediments. Nothing fancy, since I only want to verify the specificity of PCR results for primers related to marker genes...

27 May 2024 392 4 View

I am trying to resolve immune cytokines. Which is the right percentage of SDS resolving gel?

I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...

25 April 2024 9,798 0 View

Is it common practice to verify PCR results with Sanger prior to NGS?

Hello, Since a large part of my budget will go on NGS and I don't have the money to repeat it, I want to make sure that it will work. The PCR products look good on the gel (nice bands of the...

21 April 2024 7,785 3 View

How do i calculate cut off values in ELISA?

I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...

07 April 2024 3,079 1 View

How do you do rounding in APA for R Square when the value is .001?

So I know we should round the value of R Square to two decimal points, but how should you do it if the value is .001. Should you report it as R2 = .00, or R2 = .01?

26 March 2024 1,387 2 View

Sections with CMC than OCT for spatial transcriptomics slide using Visium HD?

Has anyone run slide in Visium of tissue sections (Heart, kidney, brain) with CMC than OCT for spatial transcriptomics ???

14 March 2024 243 2 View

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

I have selected two deep learning models CNN and sae for data analysis of a 1 d digitized data set. I need to justify choice of these two dl models in comparison to other dl and standard ml...

18 January 2024 6,800 1 View

Need to justify choice of two deep learning models CNN and sae compared to other models in data analysis ?

I have used two dl models CNN and sae for data analysis of 1 d digitized data set. I need to justify use of these two models in comparison to other dl and other models. I am using ga to optimize...

18 January 2024 9,816 0 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

28 July 2024 2,794 6 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,492 6 View

Is it possible to manufacture polypropylene sheets using a biaxial process?

I know that films and other low-thickness laminated products are produced using biaxial orientation, but is there something similar for sheets with a thickness of around 6 mm? Or are they...

21 July 2024 1,559 1 View

MDCI module in Orca software?

Dear Researchers, My question is associated with the "MDCI" method in Orca. Please let me clarify my question using a simple example: Suppose we are going to perform CBS extrapolation using "!...

21 July 2024 1,632 0 View

Omkar S. Kushwaha

Nanogels can be characterized by using state of the art techniques such as FE-SEM , AFM, TEM.

Some authors reported frequent use of SAXS as well as DLS.

Yongjun Men

Nanosight, a new technology.

It might also be possible to absorb some dyes, and then observe them from laser confocal microscopy.