How can I quantify butyrate of a coculture of bacteria and CRC cell lines?

12 March 2022 0 6K Report

I`m trying to do a co-culture of bifidobacterium and CaCo2 cell line, but I don`t have enough time to do Western Blot or qPCR.

Badges
Science topic

More Arlin Alfaro's questions See All

How to calculate phage concentration?

Hi all. I am trying to calculate the concentration of phages purified by the PEG method. How should I proceed? I would like to express the result in nM/ml thanks

07 August 2023 9,725 4 View

Someone have experience fixing a software bug in Fluoressence Software of a Horiba Fluorolog-3 spectrofluorimeter?

We recently moved our Fluorolog-3 equipment and after reassembling it, and when starting the Fluoresscence software we got the error described by you in your manual "Error message: Origin 8.1...

21 June 2023 3,034 0 View

I obtained these cells from a primary culture of liver of mice and I can not identify what kind of cells these are?

I did a primary culture with the objective of isolate hepatocytes, but I only obtained these cells. I need a bit of help trying to identify what kind of cells are? I took a few photos of them. I...

08 June 2023 7,890 1 View

What kind of medium and what porcentage of FBS should be used to cultivate Non-Parenquimal liver cells for a primary culture?

I need to do a primary culture of Non parenquimatic liver cells from mice and althought, I have the protocol for the obtaining and isolation of these cells, I do not know which medium to use, what...

09 May 2023 5,171 2 View

If I use a flight tunnel, what is the best way to test landing distance from a blood meal?

I am a student testing the preference of mosquitoes for feeding under different lights. I intend to use a flight tunnel with no wind, instead of a Y-tube as I have been informed that the results...

13 June 2022 1,977 0 View

Could you give some recommendations for a glutathione peroxidase assay using as samples supernatants of hemolyzed erythrocytes?

Good morning, I am Miguel Alfaro. As part of my thesis project, I am evaluating the antioxidant activity of the glutathione peroxidase enzyme (GPX) obtained from hemolyzed human erythrocytes,...

24 April 2021 3,547 4 View

Does anyone have any idea what these fibres could be?

These atmospheric particles where sampled in Mexico city using a PM10 high volume sampler. Anyone has any idea what they could be?

22 October 2018 6,656 3 View

Can I freeze at -20 C E coli suspension for posterior extraction of DNA without affect its integrity and purity?

I am using the QIAamp DNA Mini kit 250 from QiaGen for purify genomic DNA from E. coli. I have a biomass and I am looking for the best storage conditions because I am not going to perform the DNA...

06 August 2018 9,289 2 View

Ancient faults acting as huge cracks?

We observed an old strike-slip fault with schist shear damage zone about 20 m wide, with no Pleistocene-Holocene activity. Associated to this fault there are huge cracks Mode 1 opening tensile...

03 April 2018 1,289 0 View

Any paper which links submarine slump sizes with earthquake magnitudes and peak ground accelerations?

I need any graph or empirical formula which shows this. Regards.

07 August 2017 6,822 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View