Hi everyone!

I have to quantify z stack images in two different channels, one for KI67 and the other one Dcx.

I have to identify the number of cells positive for each marker and double-positive for two markers.

The problem is as we work with z stack images I don't want to lose information from any stack and if I make a stack merged, the signal overlaps and I can't quantify it individually.

On the other hand, as Dcx is diffuse cytoplasmic staining is really difficult to establish the limit between isolated cells and the stack merge is really overlapped.

Could someone help me to make this quantification automatically?