https://www.thermofisher.com/us/en/home/life-science/antibodies/antibodies-learning-center/antibodies-resource-library/antibody-methods/antibody-purification-methods.html

https://www.thermofisher.com/order/catalog/product/44897#/44897file:

View the attachment for article on purification of IgG and IgM.

Hi Suad Alkufi,

You can use immunoprecipitation followed by the affinity chromatography. I've done it before and got the high purity antibodies (>95%).

To separate IgG and IgM from the serum (from HUMAN or MOUSE serum), firstly use the protein G column, this allows you to isolate IgG from the serum, and then using protein L column (with the remaining serum sample after the protein G column), you can separate IgM.

If you have a FPLC system, you should purchase 2 types of columns (protein G and protein L columns) and following the manufacturer construction.

Below is the link of columns that I've been using. Hope it can help you.

https://www.cytivalifesciences.com/en/us/shop/chromatography/prepacked-columns/affinity-specific-groups/hitrap-protein-g-hp-antibody-purification-columns-p-03524

https://www.cytivalifesciences.com/en/us/shop/chromatography/resins/affinity-antibody/hitrap-protein-l-antibody-fragment-purification-columns-p-05666

Best luck!

You can also try an MEP Hypercel column to purify IgG - Read more from this paper: https://www.sciencedirect.com/science/article/pii/S0022030215001563

Thanks Bubacrr G Kaira for your useful answer

Column chromatography.

By using column chromatography method.

Good question

Dear Dr. thank you for sharing this excellent question..