i am working on synthesis of one of the API molecule , but in final stage i am getting impurities at RRT 0.98 & 1.02 , i don't know the structures of impurities , i tried purification in different solvents , alcohols , ketones... etc.
tried with silica gel column separation also , but due to very adjacent to product the impurities are unable to separate in column also ,
can any one help in this case,
I suggest to change the column for better separation, identify by LC/MS an then decide if you want to do prep-HPLC or other techniques.
Dear Andrei Blasko sir
i want to do it in bulk scale , not in lab scale
The most important thing to know is the nature of your impurities. As the RRT's are very similar the impurities are very similar to your API. It might be that you detect protonated forms or tautomeric forms of your API. MAybe you should purify it via salt formation
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