I tried to produce nanoparticles by caoscervation method as mentiond below:
adding 50 mg of BSA in 1 ml of buffer then stirring at 500 rpm. The dissolved protein was then transformed into nanoparticles by the continuous addition of 4 ml of absolute ethanol with a precision FPLC pump at an addition rate of 5 ml/min in a thermostatic bath
BUT the produced particles are at micro scale.
can I produce BSA with 100nm size by using coacervation?
I would take a lower concentration of BSA. You may also try to use aqueous ethanol to avoid bulky precipitates in the zone of mixing. The stirring mode during infusion of ethanol can be varied to get better results. Finally, you may filter your suspension through a sterile 0.2 mkm-filter to get nanopartices of the desired size.
thank you for your answer
i will try that tomorrow and share my results
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