Why trans protons and antiperiplanar protons have higher coupling constants in HNMR?

Why in HNMR the coupling constant of trans protons (typically ~17Hz) is higher than the cis protons (typically ~10Hz)? Also, why the antiperiplanar protons in a cyclohexane derivative have higher...

11 June 2020 4,731 4 View

Can I proceed Heck coupling on a compound in the salt form?

Can I do heck coupling reaction on a compound in salt form without turning it into a free base? As for example doing Heck coupling on p-chloropyridiniumchlroide. I don't want to turn my compound...

19 April 2020 5,593 12 View

Is 0.5 ml of DMSO enough to react my (200mg) Ar-Cl with 1.2 equivalent of primary amine using Ullman reaction?

I'm trying to react Ar-CL compound (200mg) with a primary amine (1.2 equivalent), and I've found in the literature that they're using a very low amount of solvents (like 0.5ml) to proceed the...

17 March 2020 1,016 5 View

Can I proceed N-Boc protection for an organic compound which is in the di-phosphate salt form?

My di-phosphate salt compound contains two 3ry amino groups involved in salt formation and a one 2ry amine group that's not involved in salt formation. I wanna proceed N-Boc protection for this...

15 March 2020 945 3 View

My supervisor told me my question and hypothesis are broad and too obvious, what should I do I already finished writing?

So I am writing about hate speech and chose two case studies from Denmark and France. And what I found is that hate speech is heavily influenced by nationalism but my supervisor told it is broad...

03 March 2020 658 3 View

How can I evaporate Methanol from extract by water bath as an alternative of rotavap ?

I have 10 ml methanol extract and unfortunately, my lab is lack a rotavap how can I evaporate this solvent by water bath

16 January 2020 4,897 2 View

HPLC actinomyceyes active compound ?

I have actinomycetes extraction and I need to detected by HPLC, so I need the protocol of hplc RP-8 chromatographic column

29 December 2019 7,578 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

How to choose the stimulus for T-cell activation assays?

When doing T-cells activation assay, for example looking for CD25 ,CD69 expression or ELISA for cytokine release, is there a difference between the coated antibody or coated protein stimulation...

08 November 2019 674 3 View

How can I produce BSA nanoparticles by coacervation method?

I tried to produce nanoparticles by caoscervation method as mentiond below: adding 50 mg of BSA in 1 ml of buffer then stirring at 500 rpm. The dissolved protein was then transformed into...

05 March 2019 3,941 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View