We have prepared dye bound surfactant to be used in extraction. How can I prepare surfactant bound to a dye for TEM analysis, can we follow the same protocol as we do for biological samples or is there a specific method to prepare it?
Needless to second John George Hardy's comment for OsO4 as primary "stain" in TEM. What would be of interest to us would be the combination "dye-surfactant" you created (>), what you mean with "extraction" and what the outcome of your application should be (?tissue specimens? experimental animal, human??). I could imagine there were some other "dyes" too usable for a combination with ( phospholipids, neutral lipids (cholesterol-ester) and proteins in a relation of 10:1:1, produced by pneumocytes Type II) which can be applied and examined by TEM.
You have to explain in more detail what you are trying to do. What do you mean "extraction"?
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