Sivananth Murugesan

9 Questions 13 Answers 0 Followers

Questions related from Sivananth Murugesan

How to interpret MS data for an unknown polymer ?

I have performed LC-MS for an unknown polymer sample from fermentation broth. Acetonitrile:water mobile phase of 50:50 (vol:vol %) was used. MS data analysis was performed for a single peak which...

22 March 2016 5,045 5 View

What is the % sulphuric acid to use for methanolysis of PHA?

After production of PHA when the monomer content is not known , how to analyse the purity or compound structure by doing GC-MS or HPLC; During methanolysis as shown in the paper should different...

29 June 2015 3,437 6 View

What is the retention time of lactoferrin when using TFA and acetonitrile as mobile phase in a reverse phase C18 column?

when I use C18 Reverse phase column , TFA and acetonitrile as mobile phase , gradient elution at a flow rate of 0.5 ml/min , the retention time is around 3.58 mins and I am not sure about the...

10 June 2015 8,888 3 View

What is the best way of forming soy bean lecithin based micelles?

From journals , it was clear that soy bean lecithin is good in forming micelles that can be used to deliver hydrophobic drugs when administered as oral drug delivery. what would be the best soy...

14 March 2015 6,061 2 View

How can one increase the micelle volume for cloud point extraction studies?

I have been studying cloud point extraction using different surfactants, but when I do so the micelle volume formed, that can be considered for physical parameter estimation, is very small (for a...

14 March 2015 7,505 3 View

With regards to extraction of PHA(Polyhydroxyalkanoate) - how can I confirm that all the PHA is dissolved in chloroform?

When I try extracting the PHA from biomass using chloroform, I follow the procedure as it has been described in a journal paper. I had to centrifuge the biomass and then the pellet obtained was...

19 January 2015 9,213 8 View

How can I prepare dye bound surfactant for TEM?

We have prepared dye bound surfactant to be used in extraction. How can I prepare surfactant bound to a dye for TEM analysis, can we follow the same protocol as we do for biological samples or is...

19 January 2015 1,822 2 View

How to analyse the thermogravimetric peaks to determine the PHA within the cell ?

With respect to the reference in the paper (Link) I see that the melting point is quite low for the produced PHA as a result of the carbon source being used, I am using glycerol as the carbon...

31 December 2014 3,532 1 View

How can I quantify the PHA with the Crotonic acid assay ?

I have been quantifying PHA produced by the microbes via crotonic acid assay. I had centrifuged 15 ml of medium to obtain a considerable amount of biomass , and I had treated the biomass alone...

31 December 2014 3,837 7 View