What is the difference between aptamer treated nanoparticles and functionalized nanoparticles?

03 April 2017 7,724 3 View

How to synthesize powdered gold and silver nanoparticles so that they do not agglomerate?

Colloidal solution of gold and silver nanoparticles tend to agglomerate when centrifuged.

02 March 2017 9,198 4 View

How many research papers have been published on "Smartphone based sensing devices" in the last 10 years?

I want to make a statistical collection of number of research papers published on "smartphone based sensing devices" on various fields in the last 8-10 years. How can I get the information on the...

03 April 2016 411 0 View

Can we use plano-convex lens for producing uniform illumination?

A plano-convex lens is supposed to make the light rays parallel along the optical axis of the lens. Does it ensure that the collimated beam falling on a surface gets uniformly illuminated?

03 April 2016 7,006 4 View

How does size of AuNPs affects the adsorption of BSA on the AuNPs?

31 December 2015 6,359 2 View

How can we improve the selectivity of LSPR based sensing in complex biological media?

In complex biological media, many biomolecules can have the affinity to bind with the nanoparticles' surface but when it comes to selectivity, it becomes difficult to control the selective binding...

31 December 2015 1,564 1 View

Where can I find the number of published research papers in a given area?

Can anybody help me in finding the year wise publication record on a given area of research?

31 December 2015 4,971 1 View

Apart from TEM imaging, what are other characterization technique available to ensure binding of BSA protein and Trypsin enzyme on AuNPs colloids?

What are the characterization technique available to ensure binding/ adsorption of BSA protein and Trypsin enzyme on citrate capped gold nanoparticles in colloidal form. It means I want to do the...

11 December 2015 4,093 14 View

How does sensitivity (nm/RIU) differ for LSPR in solution phase as compared to LSPR using nanoparticle substrate fabricated using lithography?

11 December 2015 971 3 View

Can we say Catalase enzyme and BSA protein as biomolecules?

Can we say that BSA protein and catalase enzyme are biomolecules?

11 December 2015 762 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View