I used this method to make concentrations, by using distilled water to make concentration like 25mg/ml
25mg of extract in 1ml of distilled water and so on ....
The most precise would be to do it in a volumetric flask, but if you don't have a small one, you can do it as mentioned.
Your question is NOT clear, Did you evaporate methanol completely to Dryness?
If yes, Is it soluble in water? I don't think so. If it is soluble, you can do as mentioned.
At first you need to prepare concentrated ethanol extract by 80 or 90 % by using rotary evaporator to gain viscus matter (oleoresin). Its a concentrated extract per g
Afterthat, you can prepar any solution w/v
Dr Eshak after i gain a concetrated extract,WHAT the next move? How to prepare stock solution
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