Hi
I would like to ask about protein precipitation by salting out method.
I'm going to precipitate collagen from fish scales. Some articles said the supernatant of the extract salted out by adding NaCl to a certain concentration, for example 2.0 M. Does it mean adding solid NaCl or NaCl solution? And how to calculate it?
Can you explain it more detail?
I've read ebook from Coligan (2001), Current Protocols in Protein Science. The book explain about salting out method and the calculation. Can I use that equation to calculate the mass of NaCl?
(The equation on the attached file)
Thanks!
Prepare concentrated NaCl solution. Add calculated amount to your collagen solution to make the final volume to 2M
Do bit of reading about calculation
http://faculty.mu.edu.sa/public/uploads/1338113774.7306projectpdf[1].pdf
Thanks for the answer Dr Murugesan.
But I can't access the link above.
For a related query, see: https://www.researchgate.net/post/Alternative_methods_to_precipitate_collagen_proteins_with_neutral_salts
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