How can I pick forward and reverse primer from the CODEHOP generated sequence?

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How can one determine the conversion factor for a radiolabeled substrate to a radiolabeled product using slopes from linear regression equation?

I am preparing a radiolabeled product using a radiolabeled substrate. I have the cpm values for different dilutions of both my known substrate and unknown product ? How can one determine the...

27 July 2024 3,604 1 View

Is it necessary to incorporate deformation energy and entropy corrections when calculating MMPBSA binding free energy in Protein-ligand complexes?

The inclusion of deformation energy and entropy corrections in MMPBSA binding free energy calculations is common in studies involving DNA-ligand complexes. However, in the literature, these terms...

25 April 2024 7,286 0 View

What is the dosage of application of nanobiofertilizer for soil application and foliar application?

Nanobiofertilizer is reported in some literatures as only samll quantity required compared to traditional ones.

27 February 2024 8,027 0 View

Is there any reliable method besides LC-MS for quantifiying Pepstatin A compound?

I tried different protein assay kits (BSA, fluorometric peptide), but it didn't work.

25 January 2024 3,341 0 View

I would like to work on complementary SRR. How to simulate CSRR using HFSS/ CST?

16 December 2023 1,658 1 View

How does technology dependence affect the development of metacognitive skills in learners?

Any thoughts on this?

23 October 2023 8,131 0 View

How to remove only water from a 6% ethanol, 4% lignin and 90% water mixture ?

can anyone help me out in the process for removing water from 6% ethanol, 4% lignin and 90% water mixture and retain ethanol and lignin alone.

27 September 2023 6,538 0 View

How to create the intermediate structures in CO2RR?

I am exploring the CO2RR pathway. While doing so i need to know the intermediate structures. How to create those intermediate structures??

13 August 2023 4,812 0 View

How to calculate Plasma Frequency using VASP?

I started with structure Optimization and then ran SCF calculation to Generate WAVECAR file. Then calculated Plasma frequency using LOPTICS=.TRUE. and following tags. is the approach correct for...

07 August 2023 7,832 1 View

How to separate Mica, Feldspar and quarts from china clay waste (filter cake)?

I have to separate mica, quart and feldspar from the china clay waste (filter cake/soil washing residue) is there any method that can be used for separation. The particle size of the sample is...

13 July 2023 3,011 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View

Where can I get PCR Primers for COI barcoding of birds?

We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...

16 July 2024 118 2 View