Ntoukakis et al. use 100uM concentration. 

Protocol:

Grow agro culture. Pellet. Resuspend in 10mM MgCl2. Add MG132 to 100uM. Infiltrate leaves.

Article Host Inhibition of a Bacterial Virulence Effector Triggers I...

Dear Helder Anderson Pinto Da Silva

Pls. find the attached files

http://www.nature.com/nature/journal/v451/n7177/full/nature06520.html

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0052546

https://www.ncbi.nlm.nih.gov/pubmed/22576093

http://www.jove.com/visualize/abstract/22576093/in-vivo-ubiquitination-assay-by-agroinfiltration

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2009.04109.x/full

http://link.springer.com/article/10.1007/s11103-015-0375-1

http://link.springer.com/protocol/10.1007%2F978-1-61779-809-2_12#page-1

http://www.plantcell.org/content/27/9/2455.full

http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-61779-809-2_12?verPrint=print

http://www.plantcell.org/content/27/9/2455.full

http://www.jove.com/visualize/abstract/22178740/synergism-between-arsenite-proteasome-inhibitor-mg132-over-cell-death

http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-61779-809-2?uri=/Abstract/doi/10.1007/978-1-61779-809-2_12

Hoping this will be helpful

Regards

Prof. Houda Kawas

Thank you so much Prof. Houda Kawas

Best Regards,

Helder Anderson

you most welcome

Houda