I want to optimize four carbon and nitrogen sources along with pH, Temp, incubation time and size of inoculum. I am using design expert version 9. Is there any option through which all of these can be optimized at one time. Kindly help me with this.
You can optimize any parameter via RSM. but it is always preferable to optimize nutrients sources (medium componenets) and physical/physiological parameters (pH. temperature, RPM) separately. so first optimize either of these two types of parameters separately through one factor at a time and then use plucket burman design and then go to other RSM techniques.
As Dr JK Saini said it is better to be optimize the physical parameters like pH and temperature should be done by one-factor-at-a-time method. Then use PBD for the carbon, nitrogen sources or mineral salts etc. There are rules for PBD matrix making, which can be downloaded from net n u can do the matrix by own also by preferring the rules. All d best.
physiological parameters are also imp. as the nutrients of production medium. Temp., rpm, pH etc are those which can be optimized first as one factor at a time den u use PBD for other sources like c source and nitro sources and also mineral salts.
yes u can use each sources separately but it is recommended to use above 4 sources for each section as PBD rule says so.
All d best...
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