Hello, I am evaluating the expression pattern of some proteins during the process of neuronal differentiation (1 day in vitro to 32 days in vitro) in primary neuron culture using western blot. I tried to normalized the results using housekeeping proteins such as b-acting. However, the housekeeping proteins time dependently increased during this period so I cannot use them as loading controls.

How can I normalized my WB results?

Thanks

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