Hello everyone, I performed an Interferon Y ELISA after a coculture of my cell lines transduced with vectors containing antigens and pbmcs transduced with 8 t cell receptors recognizing the antigens. My problem is that transduction efficiency between the tcrs is very different from 1% to 94%. I would like to analyze the Interferon y secretion on the basis of transduction efficiency, but I think I have to normalize my data. How can I do this? And what is the easiest way to do it? I am writing my master thesis and I don't have any experience in normalization yet. So it would be great, if somone has an idea. Thank you so much!
Convert your raw data for interferon gamma elisa into a 8 t cell receptor -normalized value. The normalization can be done by dividing the IFN-g values by 8 t cell receptor values. Then calculate the fold change between the different controls and treatment groups.
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