I have made a popg:pope bilayer lipid via charmm gui online for molecular dynamic purpose. But i could not pack my lipid via inflate script because it works only for ine type lipid.
I built the entire membrane protein, but I have to packing lipids around an embedded protein to analys it`s interaction with my target protein with gromacs.
I think, it's not a good idea to use InflateGRo especially when u r working on mixed lipids. I ll recommend u to use g_membed program. It is bit tricky, but I guess u can make it. The crucial thing is to input a perfect box dimension in the editconf format.
Final area per lipid could calculated by another software, as you can search in the web, like GridMat-MD depends on calculation flat membrane or Vesicle one by FATSLiM .