Hi,
I am going to measure the Na+, K+, and Cl- ion uptake by whole plants, using two plants whose shoot dry mass is 5g and 2.5g per plant.
Could you please tell me how I can measure their total ion uptake capacity? Or can you please send me any published papers?
Take one gram plant sample and digest it in diacid mixture of HNO3 and Perchloric acid. Get near dry the acid on hot plate. Make a 100 ml volume and than get the reading of different ions by AAS or any other instrument. Thanks
Take one gram plant sample and digest it in diacid mixture of HNO3 and Perchloric acid. Get near dry the acid on hot plate. Make a 100 ml volume and than get the reading of different ions by AAS or any other instrument. Thanks
Thanks everybody who suggested me in various aspects. I know the method of ion analysis from dry mass. However, I am wondering is their any published work where the entire plant's ion uptake capacity has been measured. I would like to compare the ion accumulation capacity in entire plant levels of my two tested plants in regard to salinity remediation. Thanks again.
use flame photometer to measure total Na, K and Cl from one diacid digest
If you are trying to measure total ion absoprtion, you just need to multiply "shoot dry mass" and ion concentration:
For plant #1: %Na+ * 5 g / 100 = Na+ adsorption in plant #1
digestion of plant sample, use flame photometry, express results in your sample, and you can express final results in ppm
Thanks everybody especially @ Arnoldo Gadea-Rivas, I am thinking what you suggested. Do you have any published paper so that I can use as reference or any extra suggestions?
Hi Mohammad,
Here's the papers that I think it might be useful.
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