I transduced the ETV2, ERG and FLI1 genes into iPSCs-derived brain endothelial cells. But there is no selection marker. I do not know how to be sure the transduction efficiency of these genes into the BMEC?
I was wondering if anyone has any recommendations to build .pdb files or similar for polymers in different arrangements. Specifically for PLGA. It'd be great if there's code or an easily usable...
22 January 2024 7,578 0 View
In the ongoing project focusing on the design of a Prosthetic Heart and Venous Valve, our objective is to enhance the optimization of the materials used. Our focus is to prioritize materials with...
26 October 2023 9,368 2 View
If yes, does this association fade away at time (inreaseing age)?
22 August 2023 7,198 1 View
I want the loading efficiency to be high without sacrificing the material to be encapsulated.
23 July 2023 2,649 1 View
Dear Colleagues, There has been a substantial increase in the intensity, frequency, and duration of extreme natural events (e.g., hurricanes, storms, tsunamis, and landslides) over the past four...
19 March 2023 1,882 0 View
Depression duration varies by individuals and recurrent episodes can be observed. What are the determinants of duration?
07 March 2023 7,060 3 View
I am investigating the influence of the Big Pharma in the society; some mental illnesses recently got over-diagnosed, and treatment of those mental illnesses 'happened to be' pharmaceutical. There...
28 September 2022 622 2 View
Is there an open code to make an accelerometer cooling fan project with r code?
11 May 2022 7,939 1 View
I want to see the effect of 2-DG on splenic B cell viability. But before that I want to make sure that 2-DG is working . Is there any method for me to analyze this in flow cytometer? (Is there any...
18 October 2021 7,101 0 View
Hi everyone, I have Covid infected tissues and have to isolate RNA from them. But I dont have an access to BSL3 or 4 laboratory in my institute. My colleagues are prepared and fixed tissues in...
30 June 2021 6,847 5 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View