How can I measure the thickness of rat cerebral cortex from horizontal brain parraffin sections?

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Amy Birch

Are these sagittal sections? Have you stained them with anything?

I would recommend doing Nissl or Cresyl Violet staining and then you will be able to distinguish the corpus callosum very clearly. This is a pretty good landmark for the "edge" of the cortex.

Simon Kalita-de Croft

I'd also add here that DAPI is a useful stain to use for this. Whichever stain you use, you can measure the pia to white-matter distance as a measure of cortical thickness.

If you're interested in the thickness of each layer, then staining for VGluT2 is a useful was to delineate each cortical layer. =)

Noha Bahey

Thanks for all