Determine the number of quails in a treatment/replication/pen?

How can we estimate the number of quail birds in a treatment and in a replication for 5 treatment and 4 replication for feed research? Is there any range limitation to keep the number of birds?

19 November 2020 2,856 3 View

Does any one know an integrated cloud governance framework for all levels of government (federal/state or provincial, and municipal)? Any suggestions?

How can the Social Cost Benefit-SCB Analysis be used in Cloud adoption? Perhaps, it is equally important as the Return on Investment-ROI?

04 September 2020 9,310 1 View

How it is possible that DNA primers previously working suddenly stop to worked?

I have used the same exact sequences in primers while ordering the new primers. The old primers are that I used previously which was worked successfully. Similarly, the sequences in tubes are also...

20 August 2020 3,132 12 View

Can you suggest some unconjugated primary antibodies for IHC against Pig formalin-fixed paraffin embedded(FFPE) tissue samples?

I am looking for unconjugated primary antibodies against CD3,CD4,CD8,CD11c, CD14, CD21,IgM, MHC-II in Pig FFPE tissue samples. Has anyone used Pig FFPE tissues for IHC against these markers ?

08 December 2019 1,450 3 View

Is it possible to perform Mann-Kendall Test for trend analysis of 5 years time series data?

I am having confusion and problem in trend analysis. Is it possible to perform Mann-Kendall test for trend analysis of 5 years time series data of groundwater level?

08 October 2019 733 4 View

Why PCR from cDNA is not working?

We are trying to clone a group of genes but failed at PCR step. What could be the possible reason? our protocol is briefly outlined below: 1. Extract total RNA from HEK293 cells (purity and...

07 May 2019 1,813 5 View

How is the processing of ERP signals done ?

I have collected 2 channels of EEG data with subjects looking at some visual stimulus. I have looked into a bunch of websites regarding EEG processing steps once we acquire the signal. But I have...

10 February 2019 5,989 5 View

Is there any oddball paradigm experiments written in matlab ?

I want to use oddball experiment for my EEG research. Can anyone provide any code which uses such oddball paradigm ?

10 February 2019 9,244 2 View

Is A4/Fuk cell lines metabolically active?

I have cultured A4/Fuk cell lines and it looked healthy in T75 flasks. I seeded for drug screening and growth curve. The cell count in growth curve in next day showed that there is no growth of...

06 February 2019 406 3 View

Can we do regression analysis in SPSS using the imputed data from measurement model in AMOS?

Dear Scholars I am going to test a complex model (11 latent constructs with 65 items). I did pooled measurement model in AMOS and reached good model fit with the help of modification indices. Out...

16 November 2018 6,347 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View