Hi Linda,

From protocol.com, 1.5 units = 1 Kunitz unit.  So 150 units will be 100 Kunitz units.  If you have 1 kunitz unit/µL, you'll need to add 100 µL to your working stock.

Good luck!

Thank you so much :)

Hi @ Ying Henderson ,

I am looking for the Kunitz to enzyme Units conversion, could you be more specific about the source of information for the conversion provided here: 1.5 units = 1 Kunitz unit. I could not find any other source other than this answer here, it would be more helpful to know about this conversion for me to use Rnase A in plasmid isolation.

After pondering Kunitz to Units conversation for 2 days and asking several people, I finally got an answer from a collague. Apparently Kunitz is an old unit to measure DNAse activity and it is not widely used anymore. It is not directly converted to activity U, instead they should be determined idependently. I was also instructed to use the conversion "1 Kunitz Unit is roughly 1,5 U".

Source: Dude, trust me.

I ordered from Promega DNase I enzyme. But they bought from company Omega DNase I in concentration 20 kunitz/ul. From conventer I found that 1,5 units=1 kunitz units. They write on the protocol that for 50 preps 1500 units. I can not undestood how units in one ul volume.

Hello alls

i hope someone help me

i bought sigma aldrich total rna extraction kit i want to treat my extraction with RNA free DNase from promego

In sigma kit they mentioned to this:

to prepare working stock

0.25 Kunitz unit/μL RNase-free DNase I solution

promego is measure by unit

I could no

As 1.5 units = 1 Kunitz unit.  So 150 units will be 100 Kunitz units.  If you have 1 kunitz unit/µL, you'll need to add 100 µL to your working stock.