How can I make the extraction of hemolymph efficient?

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What are the most effective remote sensing techniques and algorithms for detecting LULC changes in rapidly urban area?

What are the most effective remote sensing techniques and algorithms for detecting and analyzing land use and land cover (LULC) changes in rapidly urbanizing areas, and how do their accuracies and...

11 July 2024 6,695 1 View

Students are found bad at listening as they do pretty well in speaking tests as compared with listening tests? How to overcome this problem?

My attempt to promote listening was "Using Short-stories". It works fairly but there could be better options. Role of Short Stories in Developing English Listening Skill

09 July 2024 9,078 4 View

Using Homophones & Homographs is a challenging task for students. Can you please add your ideas and experiences to help them?

I have tried to resolve this issue a little bit, but eager to get your insights and thoughts to make my efforts better. Using Homophones and Homographs in ESL Classrooms

04 July 2024 6,742 3 View

Would you please share your thoughts and insights to bring reforms in 'Distance Education System'?

The contribution of "Distance Education" is immense, but still needs a lot of reforms. Universities generally adopt one another ideas and ignore the ideas of world wide scholars, distance...

04 July 2024 2,545 6 View

Language teachers can develop speaking skill through listening practices. Can you please suggest some more ways to promote the same (Speaking Skill)?

"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...

01 July 2024 8,406 5 View

How to develop English listening Skill among Hindi/Urdu Speaking students?

In educational institutions where English is taught as a second language, only 25% of listening happens in English but rather 75% of listening takes place in other language like Hindi/Urdu/Punjabi...

28 June 2024 6,353 4 View

How do we identify plant compounds by using thin layer chromatographic techniques?

How do we compare the Rf values of the spots to known standards or literature values to help identify the compounds. How TLC does allows the identification and analysis of the mixture of...

07 June 2024 1,967 5 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

What is the limit of penetration in carbon steel for eddy current based inspection?

Theoretically, at lower A/C excitation frequencies; say around 100 Hz, penetration of 5-10 mm is achievable in carbon steel (ferromagnetic) material. But, there is literature suggesting that this...

10 March 2024 548 0 View

How to set up a PCR protocol?

I have designed 2 primers Now I need to set up the PCR protocol. First: I need to know how much of each primer, H20 and GoTaq® G2 Hot Start Taq Polymerase to put in the mix. We usually put a...

28 February 2024 213 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View