How can I make monomers of IgM?

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Luisa Maria Villar

You should reduce interchain disulfide bonds by incubating your sample en 50 mM DTT, 0.1 M Tris/HCl, pH 9.5 during 30 minutes at room temperature. Then you can dialyse your sample and store the monomeric IgM in PBS.

Ramesh Nayak

After reduction you should alkylate to prevent re-association

Damodar Gullipalli

Check this Kit

https://www.piercenet.com/instructions/2160341.pdf

Muralidhar Katti

Can treat with 0.1M of 2-mercaptoethanol for 30min to reduce disulfide bonds and dialyse against PBS (Re: Int Arch Allergy Appl Immunol. 1984;73(4):296-302).