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Questions related from Pavel Khramtsov
I have a plate made of ABS (something like 96-well ELISA plate) with square-shaped wells. I intend to use it to do dot immunoassay. ABS is hydrophobic so buffer solutions do not spread over the...
07 July 2015 8,232 7 View
We usually use nitrocellulose with 0,45 µm from Bio-Rad. We'll immobilize antibodies to make sandwich assay. What membranes with high protein capacity do you know?
03 March 2015 9,162 3 View
Last week we adsorbed cardiolipin on polysterene microplates and nitrocellulose. We tested sera from healthy people and VDRL-positive sera. Sera from healthy people produced as a high signal as...
02 February 2015 4,959 7 View