I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of equipment are quite large and it is difficult to get the lens relatively close to the probe. I need to determine how far the antibody is travelling from the probe to determine if the antibody will affect the neurons near the lens. I know our lab has a (680XL) fluorophore antibody tag. I was thinking of trying to use that then potentially sectioning the brain and seeing if it can be detected. This is completely out of my realm of expertise and any advice would be greatly appreciated.

Thanks for your time :)