Peroxidases are classified as oxidoreductases which use for catalyzing various oxidative reactions. They isolate from various sources like plants, animals and microbes. Peroxidases have wide applications in many areas like industrial, medical and food processing. Bacillus subtilis can employe for the production of peroxidases. B.subtilis isolate from soil use the Serial dilution method. Identification of B.subtilis do by performing various staining techniques and biochemical assays.for more information ,please read these pdfs that explained how isloate and screen from soil:
but i need information related to a dye/stain that is selective for manganese peroxidase. example - for lignin peroxidase enzyme methylene blue used for qualitative screening.
Manganese peroxidase (MnP) is a hemecontaining glycoprotein that is produced by ligninolytic basidiomycetes. It requires hydrogen peroxide as an oxidant. MnP oxidizes Mn2+ to Mn3+. Mn3+ oxidizes phenolic rings to phenoxy radicals which results in the decomposition of various compounds. Native White-rot fungus (Phaner ochaete chrysosporium) Manganese peroxidase.i think, this article will be useful for you:
Screening of white-rot fungi manganese peroxidases: a comparison between the specific activities of the enzyme from different native producers