How can I interpret a strange peak in the bioanalyzer results?

More Mahnaz Kiani's questions See All

What is the latest research in the world in the field of personality and artificial intelligence?

22 May 2024 1,070 3 View

By adding ethanol to salt solution which of the following compounds will be produced?

Sodium Chloride + Ethanol 1) Sodium hydroxide + Chloroethane 2) Sodium ethoxide + Hydrogen 3) sNaCl

25 March 2024 5,065 7 View

Is it possible to extract the Histones from the already isolated proteins by Np-40 Lysis buffer?

I need to do western blot against H3K9me3 and I tested it once from my Protein extracted by Np-40 lysis buffer, I had a very nice band but the problem is the band was not at the right size (15...

11 February 2024 5,692 0 View

What physiological relation exists between the production of beta carotene and starch storage in Dunaliella salina alga under salt stress?

In D. salina in which beta carotene is accumulated throughout the cells, staining with iodine reagent will cause the whole cell to be stained. NOTE: These pictures are copyright. No reproduction...

09 September 2023 6,597 3 View

Would you please tell me some hot topics in adolescents?

hi, i'm going to work on it as my thesis.

03 August 2023 272 8 View

Using the results of one simulation in the next simulation in COMSOL?

Hello I have generated a number of ions in COMSOL inside a chamber through the Electrostatic and Charged Particle Tracing modules (the first part of the simulation). Now I am going to move the...

17 July 2023 8,912 3 View

Modeling of Thermionic Emission in COMSOL (Freeman Ion Source)?

Hello I am going to simulate a Freeman ion source in Comsol. The Freeman ion source consists of a filament, which emits electrons by heating the filament. This filament is located in a...

07 July 2023 8,884 0 View

What is Carbonic Anhydrase (CA) Activity Assay protocol?

I need to check the purified bovin carbonic anhydrase activity, can anyone let me know a simple method to check carbonic anhydrase activity?

14 December 2022 4,083 1 View

Do you have any protocol for coating Fe3O4@sio2 nanoparticles with APTES?

I want to prepare fe3o4@sio2@APTES nanoparticles to conjugate with bovine carbonic anhydrase.

28 November 2022 342 4 View

What is the maximum resistance of a conductive composite that can be used for reliable electroencephalography recording?

My goal is to record electroencephalography using a conductive carbon nanotube composite. But, I don't know how many ohms the resistance of this composite should be to provide reliable...

09 July 2022 7,566 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Jose A. Garcia-Sanz

If would be nice to have the plot. Then instead of a guess it might be an educated guess.

Best

Jose A.

Anna Git

I agree with Jose, a picture is worth a thousand words... Also, can you give some more details about the fractionation, the RNA extraction and the library prep. For example - was there any carrier added at any stage?

ZHILIAN HU

hi, Mahnaz,

Please show here the plot. Then people here can figure it out.