I'm working on mechanical APDL, modeling plate with attached resonators. I have attached spring(COMBIN14) and mass (MASS21) with the plate but it is not getting integrated. Please help me to solve this.
Hello everyone, Regarding the SDH assay calculation on a Microsoft Excel sheet, I need assistance. Using the UV spectrophotmetric approach, I conducted an in-vitro SDH experiment in the A549 cell...
21 May 2024 4,985 0 View
how to extract collagen from bones specimen?
27 April 2024 6,307 0 View
Can coulombic efficiency be greater than 100 for galvanostatic charge-discharge if the discharging time is greater than the charging time at constant currents? Is there something I'm doing wrong?
18 January 2024 244 0 View
I am trying to ligate a 3Kb band isolated insert to a 12.2 kb vector and I am having issues with transformations. I hardly see colonies on the plates and even if they are formed it is very less....
14 December 2023 9,303 0 View
Structure of NiCr LDH DFT and input file
07 November 2023 5,493 0 View
I am working on an Intrusion Detection System model. For its deployment i want to extract the network connection attributes of an Internet Connection in real time. Can you suggest me some sniffer...
23 October 2023 995 4 View
I am currently working to create an AI/ML model for non-IoC(Indicators of Compromise) based Intrusion Detection System. I am having trouble in finding out where exactly can this model be deployed....
15 October 2023 203 2 View
I was given a theme 'Satellite Oceanography', that is we need to apply Machine Learning Techniques to solve the problems that occur in Oceans that can be solved using Satellite Oceanography. I...
15 October 2023 6,683 0 View
This question addresses the practical considerations for optimizing CRISPR-mediated knock-in experiments in mammalian cells. It explores aspects like donor DNA design, homology arm length,...
13 June 2023 7,669 0 View
How to calculate parameter ∆c which represents the axial deformation at the expected buckling load for braces as per ASCE 41.
02 November 2022 2,850 0 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View