I want to induce chemical LTP and LTD in primary cortical neuron culture with glycine,KCl and NMDA,respectively,can someone share me the detailed protocols?Can I add the stocks directly to the neurobasal media or buffers(e.g.aCSF)?Thanks in advance.
I wonder if anybody knows where I could find and download some sector scan ultrasound images? I need them for doing my homework.
07 April 2024 1,764 1 View
Recently, I was working on cell biology of spinal ligement tissues, the human samples obtained from spinal surgery were easy to identify, but how can I make sure of the isolated spinal tissues...
02 April 2024 2,674 0 View
I mapped in Arcgis, where green is the study species and red is the non-study species, how do I analyse the degree of mixing and the angular scale of the species?
12 March 2024 2,549 1 View
Any suggestion on microarrays (protein/ DNA/ RNA) that measure enzymes in the phospholipid metabolism pathways? Thank you.
18 December 2023 1,366 3 View
The controlling factors of surface atmospheric pressure are obviously temperature and water vapor pressure. The relationship between atmospheric pressure and temperature is very clear, it is the...
14 December 2023 4,933 0 View
I am reaching out to seek assistance in utilizing the GAP machine learning potential that developed 2018 publication titled "Achieving DFT accuracy with a machine-learning interatomic potential:...
06 December 2023 7,597 1 View
I am currently engaged in calculating the phonon vibration frequencies of self-interstitial atom (SIA) clusters in tungsten, aiming to subsequently compute the vibrational free energy.Due to the...
24 November 2023 7,982 1 View
how to dissect and immunostain adult fat body from Drosophia?
22 November 2023 7,394 0 View
I've been having issues with viral production for the last couple of months. I was able to successfully make lentiviruses using a 2nd generation system 3 months prior and have a few aliquots...
21 November 2023 9,369 2 View
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19 November 2023 5,317 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...
07 August 2024 648 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?
04 August 2024 4,090 0 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View