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Questions related from Tang Xiaoqiang
I am working on the rRNA processing deficits with qPCR but can't get consistent results, I wonder if someone has protocols that can share with me, any feedback is greatly appreciated. I did qPCR...
22 March 2020 9,229 0 View
Now I need to determine the subcellular localization of a given lncRNA with qPCR after cellular fractionation.However,I am not sure whether same amount(e.g.1ug) or same proportion(e.g.1%) of...
27 June 2017 8,262 7 View
I am trying to establish the in vitro ubiquitination to screen the compounds,the reaction system(E1,E2,ATP and reaction buffer) is the same as generally used,and the unreacted(remaining) ATP was...
27 August 2016 3,542 3 View
Does anyone know any free tools to design antisense oligos(ASO),I need it to knockdown some nuclear lncRNA? Anyone advice is welcome.
25 May 2016 2,876 4 View
Does anyone have experimence how long can the sigma etoposide(E1383) power store at RT?And what abou the etoposide solution in DMSO?Do I have to prepare immediatly before use or can I store the...
06 May 2016 5,500 2 View
What are the subtypes of genome instability and the genome instability caused by the misrepair of DNA double-strand break in proliferating and nonproliferating cells,respectively?
23 February 2016 4,355 3 View
As stated,the doubling time for my sf9 cells were increased:the cells were seeded at the density of 106/ml and three days later,it only reached 2*106/ml with higher than 90% viability.The cells...
12 January 2016 6,831 2 View
