One way to do it is to manipulate cholesterol content by means of cyclodextrins.

Dear Prof. Barrantes,

Thank you for your suggestion.  However, I am interested in creating phospholipid asymmetry (like flipping PS/PE from inner leaflet to outer leaflet).  One way people have been inducing asymmetry is by treating cells with amphipathic chemicals (like vinblastine, chlorpromazine) but there are literature showing that these chemicals also induce endcytosis.  Hence I am looking for something which can perturb phospholipid asymmetry in live cells (in timescales of ~1-2 hours) .

With regards,

Parijat