Here are some protocols : http://bookzz.org/md5/1eeb17f557c171afdde8f5674a7a467e

Sprague dawley Rats may be a good model for breast cancer using DMBA as the inducer.Try it

7,12-Dimethylbenz[a]anthracene (DMBA, a polycyclic or polynuclear aromatic hydrocarbon, PAH, containing four fused aromatic rings), comprises an anthracene (containing planar, 3 fused aromatic rings) molecule with an additional angular benzo-ring on top of the anthracene ring, and methyl groups in para-position (at carbons-7 and -12) in the planar anthracene moiety.  In 1961, Dr Charles Huggins (Nobel Laureate in physiology/medicine) and colleagues evaluated 7,12-dimethylbenz[a]anthracene (DMBA) for is carcinogenicity in the mammary gland of rodents; this PAH was considered to be the most carcinogenic of the methylated PAHs assessed. DMBA has been widely employed as a model compound for inducing breast, skin and other cancers in rodents.  DMBA is a procarcinogen, meaning thereby that it needs metabolic activation in mammalian cells in order to generate proximate and ultimate carcinogenic metabolites.  The role of estrogens (and estrogen receptors, and hormonal status) in DMBA induced carcinogenesis is also fascinating. Incidentally, beta-estradiol molecule shares structural similarities (particularly shape) with DMBA.

HUGGINS C, YANG NC. Induction and extinction of mammary cancer. A striking effect of hydrocarbons permits analysis of mechanisms of causes and cure of breast cancer. Science. 1962 Jul 27;137(3526):257-62. PubMed PMID: 14449781.

 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC220788/pdf/pnas00226-0073.pdf

 http://cancerres.aacrjournals.org/content/32/7/1404.full.pdf

 http://press.uchicago.edu/ucp/books/book/chicago/E/bo3622967.html

 http://www.nobelprize.org/nobel_prizes/medicine/laureates/1966/huggins-lecture.pdf    “Two carcinogenic aromatics, 7,8,12-trimethyl- and 7,12-dimethyl-benz(a)anthracene (7,12-DMBA), are more efficient than all others by 10 times.”

 Medina D. Mammary tumorigenesis in chemical carcinogen-treated mice. I. Incidence in BALB/c and C57BL mice. J Natl Cancer Inst 1974;53:213-21.

Medina D, Butel JS, Socher SH, Miller FL. Mammary tumorigenesis in DMBA-treated (C57BL × DBA/2f)Fl mice. Cancer Res 1980;40:368-73. http://cancerres.aacrjournals.org/content/40/2/368.full.pdf+html

Smith GH, Teramoto YA, Medina D. Hormones, chemicals and proviral gene expression as contributing factors during mammary carcinogenesis in C3H/StWi mice. Int J Cancer 1981;27:81-6.

http://jnci.oxfordjournals.org/content/91/11/967.full.pdf+html 

Dear Majid,

Here is a Research Gate link for your perusal.

https://www.researchgate.net/post/Can_somebody_please_explain_why_methylated_PAHs_are_much_more_carcinogenic_than_the_16_EPA_PAHs

Best...

Hello,

It is actually quite simple once you have mastered the technique.  You can implant tumor chunks orthotopically into the mammary fat pad of the mice doing minor surgery.  We have a protocol if you are interested.

Best of luck,

Tahra

Thanks a lot friends.

Dear Tahra,

Would you please send for me your protocol.

Initiation and promotion with MNU

Try DMBA, it surey works in Sprague Dawley Ratsl

I can suggest you to use NMU (N methyl nitrosourea), but for Srague Dawley Rat Model.

Before all you have to consider some troubles.

1. If you use inbred rat, you can obtain good results.

2. With single dose (75 mg/kg) only, you can see first tumours within 3 mounths. 

3. Together breast cancer, you can induce renal carcinoma and a kind of skin tumour with this method.

4. İntraperitoneal single dose NMU is enough to induce mammary tumor.

5. About 50-60% of animal will have mammary tumour.

6. Another important issue, you have to induce the tumour at first estrous cycles within 4th week following to delivery of pubs. 

take it easy

TRY DMBA

Thanks

Hi,

Breast adenocarcinoma can be induced in 60 days old Sprague-Dawley female rats by daily treatment with a single dose of 20 mg of 7,12-Dimethylbenz(a)anthracene (DMBA) diluted in 1 ml soy oil given by gavage for 10 -20 weeks.