How can I identify male sterile plants in a population?

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How to completely dissolve soy lecithin?

Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...

02 August 2024 6,980 4 View

How to choose the journal?

Hello I want a suitable journal in the field of remote sensing and machine learning to be judged quickly. Thank you for your guidance Thanks

01 August 2024 1,799 4 View

Can a private laptop with 16GB RAM and Intel i5-1235U 1.3 GHz processor be used for WRF simulation with a 12*12 km domain?

I want to simulate WRF for a 12*12 km domain size. I want to run LSM and BSM models with a 5 minutes time interval. My laptop specification is 16GB RAM and Intel i5-1235U 1.3 GHz processor. Can I...

13 July 2024 2,037 0 View

Is it possible to detect levels of VWF in cell culture supernatant by immunofloursence methods that are common in pathobiologic laboraatories?

Hi all, I mean chromogenic methods.

15 June 2024 4,680 0 View

Problems with designing syber-green primers for lnc_RNA GAS5?

Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?

15 June 2024 8,055 0 View

Symmetry Analysis in Abaqus-Hypermesh, How to manage?

Hello everyone I am trying to simulate a structure in Abaqus-Hypermesh insterface, considering the half of the model due to a symmetry. The model is in 3D and I'd like to know how can I acquire...

11 April 2024 1,868 0 View

What are major coagulant factors secreted from endothelial cells?

I found vWF, PAI1 and FVIII that are produced by endothelial cells, but I think there must be more, maybe more important factors to mediate coagulation process.

24 January 2024 6,165 3 View

Hello, how can I calculate the total amonia nitrogen (NH3+NH4) from NH4-N? that's because I used kit test for NH4-N but I need to know TAN?

Finally, I want to calculate free ammonium and for this purpose I need to know TAN.

29 October 2023 3,350 3 View

Ambient Intelligence vs Internet of Things? What is Similarities and Differences?

20 October 2023 1,139 5 View

Surface roughness parameter ?

hello I want to use experimental and semi-empirical models to estimate soil moisture, but I don't have the surface roughness parameter. Is there a satellite or experimental relationship that can...

15 September 2023 687 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?

31 July 2024 8,483 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View