Hi Nilesh,

May I suggest you to check out these publications. These are quite classic methods that could be an alternative to genetic approaches.

There u go.

And this one

thank you very much 'Amine' for help. I'll definitely go through that.

I have read this paper. But I am searching for another technique rather than gene knockout.

By metabolites analysis we can say this transporter may be going to express. but in case all gene to be silence, practically it is not possible. Bcoz each enzyme has their own network.

Suggest me alternate.