How can I identify formic acid and methanol in KHCO3 solution using HPLC?

Simultenous detection is not possible. Eluents and detection are not the same. MeOH requires dilute H2SO4 as eluent and Refractive Index detection. Formic acid may Need H3PO4 or perchloric acid as eluents and for sure UV detection

Bruce Levison

You could probably do this using a Hamilton HC-75 column (Calcium Form) part number 79436, 305 x 7.8 mm, 9um particle, isocratic in 0.01M Calcium Chloride in water at a flow rate of 0.6ml/min, 5 to 10 ul injection volume using a refractive index detector see 1st link. If that doesn't work try the hydrogen form of the column, just equilibrate it into the 10mN sulfuric acid and keep everything else the same, see 2nd link. Buy the longer large diameter column since this resin has a 9um particle size. Good luck if you decide to go this route with a refractive index detector; it sometimes took up to 2 days to equilibrate the RI detector which is very sensitive to pressure and temperature changes in the LC system. If you use a vacuum degasser make sure the other lines on it are primed with water; acetonitrile and methanol in the degasser will give a negative peak in the chromatogram! Back in when I worked for the pharmaceutical industry I used this column with RI to analyze for propylene glycol in water. If I recall correctly, the 10mN sulfuric acid eluent worked for that assay.

http://www.hamiltoncompany.com/hplc-application-index/hplc-applications/formic-acid-glycerol-and-dihydroxyacetone-on-305-x-78-mm-hc75-calcium-form-pn337

http://www.hamiltoncompany.com/hplc-application-index/hplc-applications/fermentation-compounds-on-250-x-41-mm-hc75-hydrogen-form-pn205

Ade Kujore

Dear Karan,

Alternatively, you may like to use a UV/Visible detector at 215 nm, 10 mN Sulphuric acid mobile phase and the Hamilton HC-75 (H+), 250 x 4.1 mm column.

Kind regards,

Ade Kujore

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