Can anybody tell me about the basic principle behind the use of neutral and alkaline comet assay ?

In most of the research paper people used both neutral and alkaline comet assay to determine the level of DNA damage either single stranded or double stranded but the exact principle of both the...

02 August 2020 2,193 2 View

Any suggestions on what kind of statistical analysis should I do with a sample size of 100?

I have done a short survey and until now, I was able to collect only 100 responses. Not sure if collecting more would be possible. So was wondering, what kind of statistical analysis would support...

20 July 2020 9,629 12 View

After regression, how to command STATA to star above significant coefficients at 10%, 5%, and 1% levels (two-tailed) and (one-tailed)?

i am using STATA for thesting my hypotheses, what is the right command should i write to get star above significant coefficients at 10%, 5%, and 1% levels (two-tailed) and (one-tailed)? i...

09 April 2018 2,251 10 View

How would I do MM-PBSA analysis after MD simulations in GROMACS?

27 January 2016 5,610 9 View

How do I perform Pharmacokinetic study through computational softwares? Is there any online server or any freely available software for it?

I need to perform QSAR and pharmacokinetic study in silico. Please guide me with any server or software which i can freely access and which is quite reliable as per results are concerned.

17 December 2015 301 12 View

Which metals ions form complex with sodium potassium tartrate?

Can +3 cations form tartrate complexes?

25 October 2015 3,094 3 View

How might I calculate Guanidinium concentration in molecular dynamics simulation of proteins?

Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...

29 July 2015 4,437 5 View

Is it possible to study the interaction of a ligand with DNA at different pH conditions through a molecular docking approach?

A ligand showing different effect at different pH. I wanted to study the interaction of the ligand with DNA. So how will i study those at different pH environment through molecular docking...

09 October 2014 5,874 3 View

Can someone help me to estimate change in phosphate concentration in mouse macrophage cell line before and after bacterial infection?

I have used malachite green phosphate estimation kit but its sensitivity was not appropriate as per requirement.

26 May 2013 9,752 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View