How can I identify a heterologous protein through western blot if the protein is not attached to any tags?

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Afruza Zaman

Hello,

Thank you for the answer. My protein is an enzyme and I am trying to study its functionality by providing a specific substrate which should get converted into a specific product. I would like to know that even if the protein is expressed with a tag, will the tag affect the efficient function of the protein in conversion of substrate to the product

Gamal Abdel-Aziz

examine its action in case of functional protein, or do immuno ppt in case OF OTHER