7 Questions 16 Answers 0 Followers
Questions related from Afruza Zaman
I want to detect the presence of a compound in a sample through HPLC. I do not know the orientation of the compound. I have a reference standard which is a (-) isomer of the test compound. How do...
05 May 2018 3,206 4 View
I am cloning a gene in a bacterial vector which has got no tags (His-Tag, GST-Tag, etc.). Can anyone please let me know how can I perform western blot to identify the expressed untagged protein?
03 March 2018 377 2 View
The observed size of the protein which I isolated is around 12 kilo dalton bigger than the theoretical size. Please do reply
08 August 2017 1,264 8 View
If I have to evaluate the effect on a dependent variable across three treatment conditions (Control , Mild , Severe) and along three time points (0 hour, 24 hours , 48 hours) in four different...
04 April 2017 4,190 17 View
I am having four treatment (treatment A, treatment B, treatment C and treatment D). For each treatment again I have three time points. The treatments are between subject variable and time points...
11 November 2015 1,549 8 View
I am having four treatment (treatment A , treatment B, treatment C and treatment D) and I have calculated relative fold change(2^delta delta ct) values of the gene of interest using delta delta ct...
10 October 2015 509 4 View
Is it possible to carry out plant phytohormone (Jasmonic acid, Abscisic acid, Salicylic acid) Quantification in LCMS/MS without Internal standard? And if yes how far the quantification is acceptable?
09 September 2014 1,952 6 View
