How can I have a homogeneous suspensions with layered double hydroxide?

More Bahare Doustvandi's questions See All

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

What is the method of standard curve of IAA preparation?

Hi, I should prapare a standard curve of IAA and I don't know the method is. For measuring of IAA in bacteria, I culture bacteria in TSB medium and mix the supernatant of inoculated medium with...

29 June 2024 4,421 1 View

Why pet plasmids can't replicate in pseudomonas?

Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and...

25 June 2024 7,083 1 View

What plasmid is good for cloning in pseudomonas?

Hi, does anyone khonw which plasmid can we use in cloning in pseudomonas? I have to overexpress two genes in pseudomonas and I don't know which plasmid can replicate and express in pseudomonas....

24 June 2024 9,240 3 View

Baseic questions in pseudomonas cloning?

Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...

24 June 2024 5,307 2 View

What is the size band of plasmid of DH5-alpha E.coli?

I have extracted the plamid of DH5-alpha of E.coli and have done electrophoresis on 1% agarose. But it's alike DNA. Can anyone know what is the size band of its plasmid?

22 June 2024 1,589 3 View

How purity is my dna?

I have done DNA extraction but I didn't have a sharp band, is that a protein contamination?

18 May 2024 1,280 1 View

Why I get dimer in dna extraction?

Hi, I ahve tried dna extraction and when I load DNA in agarose, I have seen dimer like dimerprimer in PCR, can anyone tell me why I saw dimer in dna extraced?

07 May 2024 9,269 4 View

How much CTAB/ NACl should I add in the DNA extraction?

Presently, I am trying DNA extraction of gram negative bacteria and once, I added the mixture of CTAB / NACl after buffer extraction seperately and it was almost good, but I don't know how much I...

26 April 2024 893 0 View

How much lyzozyme reauired for dna extraction?

Hi, I am doing DNA extraction of gram negative bacteria (Pseudomonas) for three month. I want to add lyzozyme in dna extraction and I dont know how much is required. I would be grateful if anyone...

22 April 2024 8,035 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View

I want to plot calibration curve of pure mirtazapine powder using HPLC. Can someone please suggest a method without HPLC buffer as a mobile phase?

I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...

14 July 2024 1,493 3 View

What binders suggestion that I can use for zinc alloy?

I am working on powder metallurgy, and during the compaction process, the powder does not pelletize and remains in its powder form. To address this issue, I am considering using binders to improve...

13 July 2024 9,003 2 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View

Can anyone suggest what amount of powder I have to disperse in the solvent for taking the FTIR spectra?

I have to compare the FTIR of the two samples, one is in powder form and other one is the powder dispersed in IPA or water. Both are same material, so how the bond will change

09 July 2024 8,011 2 View

Is VSM measurment of NdFeB alloy powder sample require any spesific sample preparation ?

Is any specific sample preparation required to perform VSM measurement of NdFeB alloy powder to get a square-shaped M-H loop? Or is it mandatory to make magnetically aligned and sintered pellets...

09 July 2024 1,969 0 View