How can I get nanoclustres from culture media?

11 April 2019 0 6K Report

Iam trying to get AgNO3 nanocluters from hela cells ,I tried freeze thaw method culture celss for 24 hours and reated with AgNO3 for 24 hours trypnized them and then centrifuged at 5000rcf for 5 min then repeated freezed and thawed for 5 min for 5 times, seems like its fail?

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