Aqsa Qambrani

10 Questions 6 Answers 0 Followers

Questions related from Aqsa Qambrani

What is The jusitifion for using cancer cells-derived EVs to deliver drug,?

These exosomes from cancer cells can cause issues relate to immune response, detrimental effect (due to functional exosomes).

03 July 2020 9,000 0 View

Iam trying to check floiurescence in bacteria, but iam failed?

I am working on E.coli bacteria after growing bacteria in LB I centrifuge it at 7000 then resuspend it and add 2 ml deionized sterile water and 19 microliters gold. In the confocal machine, there...

15 November 2019 2,957 3 View

I want to compare my insitu Synthesized AgNCs Graph with literature. But can not find any litrature?

Ihave synthsized AgNCs in Situ in Hela Cells , I have took FTiR graph but can not find any literature. How can I know if my graph is correct

25 April 2019 8,948 0 View

How can I get nanoclustres from culture media?

Iam trying to get AgNO3 nanocluters from hela cells ,I tried freeze thaw method culture celss for 24 hours and reated with AgNO3 for 24 hours trypnized them and then centrifuged at 5000rcf for 5...

11 April 2019 6,457 0 View

How much concentration of Ag,DNA , and doxorubicin i should for MTT assay?

Hi there, I want to do MTT assay of T cells and Hela cells , how much concentration of Ag,DNA and Doxorubicin i should add per well in 96 well plate?

31 January 2019 880 0 View

Hi , I wanted to collect exosomes from cell culture media through ultracentrifugation.but i couldn't see pallet. Why?

I collected media in two bottles and centrifuge for 800g RCF for 10 minutes . i couldn't see any pallet , then i went for 2000g for 20 minutes still nothing what should be the possible reason?

28 December 2018 5,777 3 View

I want to collect blood of normal mouse after treating it with Ag to check drug uptake, What is best way to collect blood?

doing animal model, for cancer related research

20 December 2018 2,746 3 View

How to take perfect confocal images of U87 hepG2 ?

After treating cells with doxorubicin DNA and AgNO3, when i add PFA to fix cells, they change their shape and images are not fair enough. what should i use to fix cells other then PFA.

14 November 2018 337 2 View

I fabricated GNP by reduction of sodium citrate but its colour is not bright red ,, what might be the reason ?

Fabrication of GNP

29 May 2018 9,853 1 View

Why Pi is used in fracture mechanics?

I see a lot of equations work very well without Pi then it is needed to use Pi in fracture machines.

01 January 1970 809 1 View