extraction techniques
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seed for cultivation of ginkgo biloba plan
The message I got from the computer was "vnmr2ucsf couldn't find the transmitter frequency for nucleus 1H in the procpar file". Please suggest the solution for this error.
08 January 2020 7,945 1 View
How we can do data collection and analysis in the form of tabulation, statistical techniques and interpretation of those results.
07 January 2020 635 6 View
I am working on E.coli bacteria after growing bacteria in LB I centrifuge it at 7000 then resuspend it and add 2 ml deionized sterile water and 19 microliters gold. In the confocal machine, there...
14 November 2019 2,774 3 View
I need to do a Pre and Post Reading Survey for a Motivational and Self-Help Novel. A Ready Made Questionnaire shall help. The same will be asked before they read and then after they have read. The...
05 October 2019 3,312 2 View
Hi, I have seen many papers where the authors have deduced the molecular weight of protein/ compound directly from the LCMS spectra peaks. I also have an LCMS spectra but I am new to this...
16 September 2019 7,014 1 View
For most carbon materials (Graphene, Graphene oxide, graphite oxide etc). we have combination of D-band (1350 cm-1), G-band (1580 cm-1) and G*-band (2700 cm-1) peaks of different ratios. For the...
02 July 2019 4,816 2 View
Hi Everyone, I am working on some drugs for HSV. I am wondering what are the best heart, liver and kidney cells lines to evaluate cytotoxicity of my drug on heart, liver and kidney...
17 June 2019 7,591 2 View
I collected media in two bottles and centrifuge for 800g RCF for 10 minutes . i couldn't see any pallet , then i went for 2000g for 20 minutes still nothing what should be the possible reason?
27 December 2018 5,642 3 View
doing animal model, for cancer related research
19 December 2018 2,428 3 View
I am facing problem regarding purification of anabaena. i have used serial dilution and plate streaking method. But everytime the culture is contaminated with chlorella vulgaris. I have to perform...
12 November 2018 7,869 3 View
Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...
26 February 2021 10,014 3 View
Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...
25 February 2021 2,442 1 View
Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...
22 February 2021 7,127 4 View
I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...
17 February 2021 8,933 4 View
Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...
17 February 2021 1,434 13 View
biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...
15 February 2021 1,102 5 View
Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...
11 February 2021 6,683 6 View
1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.
04 February 2021 9,069 3 View
I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...
04 February 2021 9,152 4 View
the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...
03 February 2021 3,198 7 View