extraction techniques
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seed for cultivation of ginkgo biloba plan
I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.
18 July 2024 4,218 0 View
The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...
01 July 2024 2,356 6 View
I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...
01 July 2024 1,841 3 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,609 3 View
I made a primer that gives 2 bands in every 15 samples in ZS11xSiloga BC3F1 genration. I thought that it might be technical problem, i again ccheck it with BC2F1 generation so i got homozygous...
21 March 2024 9,382 0 View
Actually, I am trying for months to make a primer of a Clubroot resistance gene (CRa). CRa gene is found in three Brassica species. My aim is to make a primer that can amplify this one gene in all...
06 March 2024 9,877 4 View
Some studies have shown coffee and other caffeinated beverages can reduce iron absorption. For example. Drinking a cup of coffee while eating a hamburger can reduce iron absorption by up to 39%....
26 December 2023 3,278 2 View
Autologous donations are blood donations that individuals give for their own use – for example, before a surgery.
26 December 2023 4,494 2 View
IgG is the only class of immunoglobulin capable of crossing the placenta.
29 October 2023 7,735 2 View
I hope this message finds you well. I am currently working on a research project focused on MD simulation study of drug desorption behavior on a boron nitride nanosheet with a non-covalent polymer...
28 September 2023 4,514 0 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,145 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,361 3 View