I am working on a project involving parthenogenesis in crested geckos (Correlophus ciliatus), and I plan on comparing genes- particularly STRs- in samples from the eggs, the potential parents, and previous records (via GenBank, etc), to each other. However, I do not know how to successfully isolate DNA from a very early embryo in a shelled reptile egg. I will likely have to do this, because all the previous parthenogenic eggs from my geckos have failed long before complete development. Please excuse my phrasing, this is not a subject I have much experience in yet.
Dear Margalit Sarah Kaufman go through the following link, it may be helpful for you.
Article Using eggshell membranes as a DNA source for population gene...
Hi Margalit,
The following two links might be useful for you. All the best.
https://wiki.xenbase.org/xenwiki/index.php/Extraction_of_DNA_from_Single_Embryos_(Sive_et_al._2000)
https://www.tandfonline.com/doi/abs/10.1080/00071668.2012.760035?journalCode=cbps20
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