In a general sense, what are various ways to improve the quality of ELISA experiments and their subsequent data?

Hello, I am planning to run a series of ELISA experiments and am asking for basic tips for improving the quality and reproducibility of my experiments. I have only done the experiment once, but...

01 February 2019 4,660 2 View

Has anyone gotten a clean doublet when using the Abcam anti-ferritin heavy chain antibody ab65080?

I am using the antibody for western blot analysis of brain tissue homogenates on a 12% gel. Attached is the image of the blot. GAPDH is used as a loading control and is shown around 35 kDa. The...

09 October 2018 2,175 0 View

How do I determine a proper loading control for my western blot assay using whole tissue homogenates?

I am running a western blot assay using whole tissue homogenates. The homogenates are from collected mouse brains and I am using two groups an Ames dwarf mouse and a wild-type mouse. I see that...

09 October 2018 1,473 3 View

Has anyone used commercial primers for drosophila e-cadherin? Can I use human?

I have been working through a ChIP-qPCR experiment and am hoping to run a comparative Ct qPCR analysis. I want to use e-cadherin as a housekeeping gene, but realized the primers from my lab are...

06 July 2018 2,411 1 View

When running ChIP assays can you store samples after incubation with Ab and washes for indiscriminate binding?

Can you store immuno-protein complexes prior to DNA isolation or does this experiment need to be run to completion? I only ask because for my protocol there are several incubation periods of 2-4...

04 May 2018 6,830 4 View

When writing a manuscript, where should citations go?

I often see them in the middle of the sentence and sometimes at the end, as a collective. I am wondering is there a rule for this type of citation. Is it personal preference or journal specific?

03 April 2018 7,832 8 View

When running qPCR, what is an acceptable SD for your Ct values?

I have had a variable Standard Deviation of my Ct values for my last few PCR reads. Some SD ~5 and some nearing 10. I am wondering what is considered a large SD and what is acceptable

03 April 2018 5,107 3 View

QPCR: When analyzing my result, the amplification plots of my samples is a jagged line. What could explain this?

I am running qPCR with gDNA. Using primers tested in a previous experiment, I am not seeing any exponential amplification. In fact, most samples (unknowns and my standards) return with an...

03 April 2018 9,384 8 View

How can one verify pipetting volume of a pipetman?

While running qPCR and using small volumes, I want to ascertain that I am pipetting accurate volumes. I have observed some large variation in my sample quantities.

03 April 2018 1,777 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View