I am running a western blot assay using whole tissue homogenates. The homogenates are from collected mouse brains and I am using two groups an Ames dwarf mouse and a wild-type mouse.
I see that in the literature several groups are using B-actin and not GAPDH.
Why would one choose GAPDH vs B-actin? Does someone have a resource with rationale for either loading control?
Hi Cody,
Choice of loading control depends upon origin of protein mix to be resolved and the target. For whole tissue homogenate both GAPDH and beta actin can be used. Second, if target protein size should be different from loading control (preferably) so that you can probe both the proteins at the same time.
See links below for further reading
Article Western Blot: Technique, Theory and Trouble Shooting
https://info.gbiosciences.com/blog/the-advantages-of-loading-controls-in-western-blotting
Best wishes
You can use both of them for mouse brain homogenate. But, you should not use beta actin for heart tissue because it contains a lot of amount beta actin.
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