I think firstly you should finish the dormancy. The second is you germinate seeds in basal MS medium or 1/2 MS media.

You can use 50 ml glass jar(in 20 ml medium).

and you should try best sterilisation method for raspberry.

Use 1/2 MS with MES (0.5 g/L), 10 g/L sucrose at pH 5.6. To sowing seeds I recommend 0.1% agarose with p1000 micropipette. To scarify seeds, first sterilize using NaClO 10% v/v (bleach 10%) 5 mins and three washes with sterilized water, put seeds in sterilized water at 4 ºC during two hours and sowing. If you have low viability, add 5-10 mg/L of GA3 with 0.22µm syringe filter.

After breaking dormancy you can surface sterilize seeds. To do so you can take seeds in thin cotton cloth and tie it by using elastic band then sterilize using neutral detergent+alcohol+sodium hypo chloride/mercuric chloride. After ccompleting sterilization pprocess put these seeds on MS medium without growth regulators.

do you mean i should put it in cotton clot Dry or wet?

Dry cotton cloth/ surgical gauge cloth any one.

thanks

Another technique is you can put seeds on filter paper and make it wet by using distil water and keep it on petridish. But be careful dont use to much water, just make filter paper wet. You should maintain those in incubator with 20-23 degree temperature.

thanks Ara

Seed germination is not the primary method of reproduction in Raspberry or strawberry, but rather by rhizome basal buds. Seeds are primarily a method of colonization of new ecosystems.

In my experience, while the seeds do not require a specific 'dormancy regimen', but benefit from cold, water and soil acids to break down seed coatings (think plant life cycle). I store all mature seed in the refrigerator at 4*C and removing required quantities for germination trials.

I prefer agar infused TC media in magenta boxes for germination as they are sterile, maintain moisture and are easy to handle and will hold 5 seeds with space for individual seed contamination without cross contamination.

Most issues I have found involve the sterilization process. Seeds with 'husks' cause the most problems and removing them is the best insurance against residual bacteria after sterilization procedures. The use of a shaker table is most helpful in this regard. Before sterilization, the seeds should be well hydrated (soaked in DI water for 12 hours prior). Clean the seed with ‘twiss’ or sanitizing soap solution and agitate for 15 min., rinse thoroughly and soak in 1% bleach solution again while agitating for an additional 15 min. Rinse thoroughly and soak in 70% ethanol for 1 min and rinse with autoclaved DI water and allow to continue hydrating 1-2 hours before planting with warm 23*C water while continuing to agitate, rinse again before use.

The use of a laminar flow hood, sterile instruments, and sterile technique are required. The Magenta boxes should be prepared the day before. Note: do NOT use sugar in the media as it is a food source for contamination during seed germination. Magenta boxes should be keep at 17-23*C and under low light conditions until germination occurs (3-10 days). At this time (appearance of 2 true leaves), seeds should be transferred to grow up medium.

Thanks a lot Mr Hopkins